It is estimated that over 176 million women are affected by this condition globally, with women of reproductive age being more frequently affected 1, 2. This high-visibility study is capable of directing different experimental designs as MenMSCs are derived from a minimally invasive tissue source with multifunctional roles in regenerative medicine.Įndometriosis is a benign estrogen-dependent gynecologic disease characterized by endometrium-like tissue outside of the uterine cavity. Furthermore, we suggest avoiding the commonly used GAPDH and ACTB reference genes as they are unstable. Using the RefFinder web tool, we recommend the EIF2B1 and POP4 reference genes for the normalization of RT-qPCR data in study designs similar to ours. We tested for the first time the stability of 32 candidate reference genes to achieve increased accuracy and reliable results in the quantification of gene expression and direct future experiments using reverse transcription-quantitative PCR (RT-qPCR) in MenMSCs for endometriosis studies.
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Therefore, in this exploratory study, we used stringent case and control selection criteria and collected menstrual blood from women with a laparoscopic diagnosis of advanced endometriosis and from fertile women without endometriosis.
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However, reference gene stability for proper reproducible normalization in the analyses of the expression data validation is still unexplored in this experimental context.
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It has been suggested that menstrual blood-derived mesenchymal stem/stromal cells (MenMSCs) are associated with the etiopathogenesis of endometriosis and considerable effort has been invested in searching for target genes and deciphering associated molecular pathways.